陈 肖¹，曾维艳¹，王 翔¹，孙丰慧^1,2，代 敏^2*，蒲忠慧^1,2*

（1.成都医学院检验医学院，四川成都 610500；

2.四川省动物源性食品兽药残留防控技术工程实验室，四川成都 610500）

摘要：比较草果中两种不同多酚类体外抗氧化活性。以 Vc 为阳性对照，采用体外抗氧化活性方法，测定草果中不同多酚类黄酮和酚酸对DPPH·、ABTS⁺·、·OH、O₂·^-清除能力和 Fe³⁺还原力。结果表明，草果中不同多酚类在0.625 mg/mL 时对DPPH·清除率均达到80%以上，还具有一定的ABTS^＋·、·OH、O2·^-清除作用和 Fe³⁺还原作用。其清除效果与草果多酚浓度具有剂量依赖性。草果酚酸对 DPPH·清除能力优于草果黄酮；草果黄酮对ABTS⁺·、·OH、O2·^-清除能力和Fe3＋还原能力均强于酚酸。

关键词：草果；黄酮；酚酸；抗氧化；体外

中图分类号：TS264.3      文献标识码：A      文章编号：1674-506X（2023）03-0043-0005

Study on in Vitro Antioxidant Activities of Polyphenols from Amomum tsao-ko

CHEN Xiao¹, ZENG Weiyan¹, WANG Xiang¹, SUN Fenghui^1,2, DAI Min^2*, PU Zhonghui^1,2*

(1.School of Laboratory Medicine, Chengdu Medical College, Chengdu Sichuan 610500, China;

2.Sichuan Provincial Engineering Laboratory for Prevention and Control Technology of Veterinary Drug Residue in Animal-origin Food,Chengdu Sichuan 610500, China)

Abstract：To evaluate in vitro antioxidant activities of two different polyphenols from Amomum tsao-ko, and to lay a foundation for the research and development of antioxidant chemical components in the A. tsao- ko.Through in vitro antioxidant activity tests, taking ascorbic acid as a positive control, the antioxidant activity ofthe two species of polyphenolic flavonoids and phenolic acids from A. tsao-ko on DPPH·, ABTS⁺·, hydroxyl radical, O²-and Fe³⁺reducing ability were investigated. The scavenging rates of two different polyphenols at the concentration of 0.625 mg/mL on DPPH radical were all over 80%,they also had certain scavenging effect on ABTS⁺ radical, hydroxyl radical, superoxide anion radical and Fe³⁺ reduction. The antioxidant effects were positively correlated with the polyphenol concentration within a certain range. Comparatively, the DPPH radical scavenging ability of phenolic acids from A.tsao-ko was better than that of flavonoids, the flavonoids based on the capability to remove ABTS⁺ radical, hydroxyl radical, superoxide anion radical and Fe³⁺ reducing capacity was stronger than that of phenolic acids.

Keywords: Amomum tsao-ko; flavonoids; phenolic acids; antioxidant activity; in vitro

doi: 10.3969/j.issn.1674-506X.2023.03-007

收稿日期：2022-09-29

基金项目：国家自然科学基金项目（82204601，31970137，82102442）；四川省科技厅面上项目（2023NSFSC0172）；成都医学院丽妍工作坊医美研究中心专项项目（2022YM006）；国家级大学生创新创业训练计划项目（202213705013）

作者简介：陈肖（2001-），男，本科生。研究方向：中药质量分析。

*通信作者：蒲忠慧（1982-），女，博士，副教授。研究方向：中药药效物质基础研究；代敏（1974-），博士，教授。研究方向：中药抗耐药菌机制研究。

引用格式:陈肖,曾维艳,王翔,等.草果中不同多酚类体外抗氧化活性研究[J].食品与发酵科技,2023,59(3):43-46,75.